Vesiclepedia: EVQuant results

Results shown for selected experiment:

Experiment ID 47

PubMed ID 34108659

Identification method Protein

Extracellular vesicle type Exosomes

MISEV Small EVs

Species Homo sapiens

Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker

Sample Pancreatic cancer cells

Sample name PANC-1 - Exo-rich fractions 7-10 pooled

Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography

Methods used in the study Western blotting
Mass spectrometry

Flotation density -

Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.

Journal name Nat Cell Biol

Publication year 2021

EV-TRACK -

   Contents identified in selected experiment:

   Top 200 abundant gene(s) for selected experiment:

   Other experiments from same study (PubMed ID):

PubMed ID Species Experiment description Sample Sample name Identifications Isolation methods Vesicle type MISEV Quantification

1 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Pancreatic cancer cells PANC-1 - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography Exosomes Small EVs EVQuant

2 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Breast cancer cells MDA-MB-231 - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography Exosomes Small EVs EVQuant

3 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Breast cancer cells MDA-MB-231 - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation Exosomes Small EVs EVQuant

4 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Pancreatic cancer cells PANC-1 - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation Exosomes Small EVs EVQuant

5 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Embryonic kidney cells HEK293T - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography Exosomes Small EVs EVQuant

6 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Embryonic kidney cells HEK293T - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation Exosomes Small EVs EVQuant

   Experiments in which MANF is identified:

PubMed ID Species Experiment description Sample Sample name Identifications Isolation methods Vesicle type MISEV Quantification

1 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Smooth muscle cells Carotid artery-derived primary smooth muscle cells Protein Differential centrifugation
Ultracentrifugation Extracellular vesicles EVs EVQuant

2 32487994 Homo sapiens Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence Plasma Healthy donors Protein Centrifugation
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

3 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Pancreatic cancer cells PANC-1 - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography Exosomes Small EVs EVQuant

4 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Breast cancer cells MDA-MB-231 - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography Exosomes Small EVs EVQuant

5 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Valvular interstitial cells Aortic valve-derived primary valvular interstial cells Protein Differential centrifugation
Ultracentrifugation Extracellular vesicles EVs EVQuant

6 32487994 Homo sapiens Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence Plasma Plasmodium vivax (Malaria) patients Protein Centrifugation
Size exclusion chromatography Extracellular vesicles Small EVs EVQuant

7 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Breast cancer cells MDA-MB-231 - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation Exosomes Small EVs EVQuant

8 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Pancreatic cancer cells PANC-1 - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation Exosomes Small EVs EVQuant

9 33661579 Homo sapiens Pannexin-1 channel regulates nuclear content packaging into apoptotic bodies and their size T lymphoblast Jurkat T cells Protein Differential centrifugation Apoptotic bodies Large EVs EVQuant

10 33661579 Homo sapiens Pannexin-1 channel regulates nuclear content packaging into apoptotic bodies and their size T lymphoblast Jurkat T cells Protein Differential centrifugation Apoptotic bodies Large EVs EVQuant

11 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Embryonic kidney cells HEK293T - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography Exosomes Small EVs EVQuant

12 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-KO-LAMP2A Protein Differential centrifugation
Filtration
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

13 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Smooth muscle cells Carotid artery-derived primary smooth muscle cells Protein Differential centrifugation
Ultracentrifugation Extracellular vesicles EVs EVQuant

14 33661579 Homo sapiens Pannexin-1 channel regulates nuclear content packaging into apoptotic bodies and their size T lymphoblast Jurkat T cells Protein Differential centrifugation Apoptotic bodies Large EVs EVQuant

15 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Valvular interstitial cells Aortic valve-derived primary valvular interstial cells Protein Differential centrifugation
Ultracentrifugation Extracellular vesicles EVs EVQuant

16 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-Cas9-WT Protein Differential centrifugation
Filtration
Ultracentrifugation Small extracellular vesicles Small EVs EVQuant

17 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Embryonic kidney cells HEK293T - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation Exosomes Small EVs EVQuant

	PubMed ID	Species	Experiment description	Sample	Sample name	Identifications	Isolation methods	Vesicle type	MISEV	Quantification
1	34108659	Homo sapiens	Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker	Pancreatic cancer cells	PANC-1 - Exo-rich fractions 7-10 pooled	Protein	Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography	Exosomes	Small EVs	EVQuant
2	34108659	Homo sapiens	Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker	Breast cancer cells	MDA-MB-231 - Exo-rich fractions 7-10 pooled	Protein	Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography	Exosomes	Small EVs	EVQuant
3	34108659	Homo sapiens	Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker	Breast cancer cells	MDA-MB-231 - Exo-rich fractions 1-6 pooled	Protein	Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation	Exosomes	Small EVs	EVQuant
4	34108659	Homo sapiens	Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker	Pancreatic cancer cells	PANC-1 - Exo-rich fractions 1-6 pooled	Protein	Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation	Exosomes	Small EVs	EVQuant
5	34108659	Homo sapiens	Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker	Embryonic kidney cells	HEK293T - Exo-rich fractions 7-10 pooled	Protein	Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography	Exosomes	Small EVs	EVQuant
6	34108659	Homo sapiens	Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker	Embryonic kidney cells	HEK293T - Exo-rich fractions 1-6 pooled	Protein	Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation	Exosomes	Small EVs	EVQuant