Results shown for selected experiment:


Experiment ID39
PubMed ID 33991177   
Identification method Protein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
SpeciesHomo sapiens
Experiment descriptionCancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
SampleGastric cancer cells
Sample nameMKN1 - 100K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Methods used in the studyWestern blotting
Mass spectrometry
Flotation density-
AuthorsCarli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year2021
EV-TRACK -



   Contents identified in selected experiment:





   Top 200 abundant gene(s) for selected experiment:




   Other experiments from same study (PubMed ID):



 PubMed IDSpeciesExperiment descriptionSampleSample nameIdentificationsIsolation methodsVesicle typeMISEVQuantification
1 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation		 Small extracellular vesicles Small EVs EVQuant
2 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation		 Small extracellular vesicles Small EVs EVQuant
3 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation		 Small extracellular vesicles Small EVs EVQuant



   Experiments in which S100A6 is identified:



 PubMed IDSpeciesExperiment descriptionSampleSample nameIdentificationsIsolation methodsVesicle typeMISEVQuantification
1 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation		 Small extracellular vesicles Small EVs EVQuant
2 32487994 Homo sapiens Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence Plasma Healthy donors Protein Centrifugation
Size exclusion chromatography		 Extracellular vesicles Small EVs EVQuant
3 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation		 Small extracellular vesicles Small EVs EVQuant
4 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Pancreatic cancer cells PANC-1 - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation 		Exosomes Small EVs EVQuant
5 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control129 Protein Centrifugal ultrafiltration
Size exclusion chromatography		 Extracellular vesicles Small EVs EVQuant
6 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE128 Protein Centrifugal ultrafiltration
Size exclusion chromatography		 Extracellular vesicles Small EVs EVQuant
7 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Valvular interstitial cells Aortic valve-derived primary valvular interstial cells Protein Differential centrifugation
Ultracentrifugation		 Extracellular vesicles EVs EVQuant
8 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Breast cancer cells MDA-MB-231 - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography		 Exosomes Small EVs EVQuant
9 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE130 Protein Centrifugal ultrafiltration
Size exclusion chromatography		 Extracellular vesicles Small EVs EVQuant
10 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Valvular interstitial cells Aortic valve-derived primary valvular interstial cells Protein Differential centrifugation
Ultracentrifugation		 Extracellular vesicles EVs EVQuant
11 32487994 Homo sapiens Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence Plasma Plasmodium vivax (Malaria) patients Protein Centrifugation
Size exclusion chromatography		 Extracellular vesicles Small EVs EVQuant
12 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-Cas9-WT Protein Differential centrifugation
Filtration
Ultracentrifugation		 Small extracellular vesicles Small EVs EVQuant
13 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Pancreatic cancer cells PANC-1 - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography		 Exosomes Small EVs EVQuant
14 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Embryonic kidney cells HEK293T - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography		 Exosomes Small EVs EVQuant
15 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE131 Protein Centrifugal ultrafiltration
Size exclusion chromatography		 Extracellular vesicles Small EVs EVQuant
16 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Smooth muscle cells Carotid artery-derived primary smooth muscle cells Protein Differential centrifugation
Ultracentrifugation		 Extracellular vesicles EVs EVQuant
17 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Breast cancer cells MDA-MB-231 - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation 		Exosomes Small EVs EVQuant
18 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE127 Protein Centrifugal ultrafiltration
Size exclusion chromatography		 Extracellular vesicles Small EVs EVQuant
19 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-KO-LAMP2A Protein Differential centrifugation
Filtration
Ultracentrifugation		 Small extracellular vesicles Small EVs EVQuant
20 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control126 Protein Centrifugal ultrafiltration
Size exclusion chromatography		 Extracellular vesicles Small EVs EVQuant
21 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Embryonic kidney cells HEK293T - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation 		Exosomes Small EVs EVQuant
22 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control130 Protein Centrifugal ultrafiltration
Size exclusion chromatography		 Extracellular vesicles Small EVs EVQuant
23 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Smooth muscle cells Carotid artery-derived primary smooth muscle cells Protein Differential centrifugation
Ultracentrifugation		 Extracellular vesicles EVs EVQuant
24 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE129 Protein Centrifugal ultrafiltration
Size exclusion chromatography		 Extracellular vesicles Small EVs EVQuant
25 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation		 Small extracellular vesicles Small EVs EVQuant
26 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control127 Protein Centrifugal ultrafiltration
Size exclusion chromatography		 Extracellular vesicles Small EVs EVQuant