Results shown for selected experiment:


Experiment ID28
PubMed ID 35333565   
Identification method Protein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
SpeciesHomo sapiens
Experiment descriptionLAMP2A regulates the loading of proteins into exosomes
SampleRetinal pigment epithelial cells
Sample nameARPE-19-KO-LAMP2A
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Methods used in the studyWestern blotting
Mass spectrometry
Flotation density-
AuthorsFerreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year2022
EV-TRACK -



   Contents identified in selected experiment:





   Top 200 abundant gene(s) for selected experiment:




   Other experiments from same study (PubMed ID):



 PubMed IDSpeciesExperiment descriptionSampleSample nameIdentificationsIsolation methodsVesicle typeMISEVQuantification
1 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-Cas9-WT Protein Differential centrifugation
Filtration
Ultracentrifugation		 Small extracellular vesicles Small EVs EVQuant
2 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-KO-LAMP2A Protein Differential centrifugation
Filtration
Ultracentrifugation		 Small extracellular vesicles Small EVs EVQuant



   Experiments in which IGFBP7 is identified:



 PubMed IDSpeciesExperiment descriptionSampleSample nameIdentificationsIsolation methodsVesicle typeMISEVQuantification
1 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation		 Small extracellular vesicles Small EVs EVQuant
2 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control126 Protein Centrifugal ultrafiltration
Size exclusion chromatography		 Extracellular vesicles Small EVs EVQuant
3 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE130 Protein Centrifugal ultrafiltration
Size exclusion chromatography		 Extracellular vesicles Small EVs EVQuant
4 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation		 Small extracellular vesicles Small EVs EVQuant
5 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation		 Small extracellular vesicles Small EVs EVQuant
6 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE127 Protein Centrifugal ultrafiltration
Size exclusion chromatography		 Extracellular vesicles Small EVs EVQuant
7 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-KO-LAMP2A Protein Differential centrifugation
Filtration
Ultracentrifugation		 Small extracellular vesicles Small EVs EVQuant
8 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE129 Protein Centrifugal ultrafiltration
Size exclusion chromatography		 Extracellular vesicles Small EVs EVQuant
9 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE131 Protein Centrifugal ultrafiltration
Size exclusion chromatography		 Extracellular vesicles Small EVs EVQuant
10 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control129 Protein Centrifugal ultrafiltration
Size exclusion chromatography		 Extracellular vesicles Small EVs EVQuant
11 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE128 Protein Centrifugal ultrafiltration
Size exclusion chromatography		 Extracellular vesicles Small EVs EVQuant
12 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control127 Protein Centrifugal ultrafiltration
Size exclusion chromatography		 Extracellular vesicles Small EVs EVQuant
13 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-Cas9-WT Protein Differential centrifugation
Filtration
Ultracentrifugation		 Small extracellular vesicles Small EVs EVQuant
14 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control130 Protein Centrifugal ultrafiltration
Size exclusion chromatography		 Extracellular vesicles Small EVs EVQuant