Gene description for Hspd1
Gene name	heat shock protein family D (Hsp60) member 1
Gene symbol	Hspd1
Other names/aliases	Hsp60 Hspd1-30p
Species	Rattus norvegicus
Experiment description of studies that identified Hspd1 in Cardiomyocytes
1 Experiment ID 831 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - exosomes exposed to 65.1 mM ethanol - Rep 1 Isolation/purification methods Differential centrifugation Filtration ExoQuick Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%
2 Experiment ID 832 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - exosomes exposed to 65.1 mM ethanol - Rep 2 Isolation/purification methods Differential centrifugation Filtration ExoQuick Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%
3 Experiment ID 811 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - exosomes incubated at 4°C Isolation/purification methods Differential centrifugation Filtration ExoQuick Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%
4 Experiment ID 812 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - exosomes incubated at 25°C Isolation/purification methods Differential centrifugation Filtration ExoQuick Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%
5 Experiment ID 813 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - exosomes incubated at 37°C Isolation/purification methods Differential centrifugation Filtration ExoQuick Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%
6 Experiment ID 814 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - exosomes incubated at 42°C Isolation/purification methods Differential centrifugation Filtration ExoQuick Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%
7 Experiment ID 815 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - control Isolation/purification methods Differential centrifugation Filtration ExoQuick Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%
8 Experiment ID 816 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - exosomes exposed to 21.7 mM ethanol Isolation/purification methods Differential centrifugation Filtration ExoQuick Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%
9 Experiment ID 817 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - exosomes exposed to 65.1 mM ethanol Isolation/purification methods Differential centrifugation Filtration ExoQuick Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%
10 Experiment ID 818 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - exosomes at pH 6.6 Isolation/purification methods Differential centrifugation Filtration ExoQuick Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%
11 Experiment ID 819 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - exosomes at pH 7.1 Isolation/purification methods Differential centrifugation Filtration ExoQuick Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%
12 Experiment ID 820 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - exosomes at pH 7.4 Isolation/purification methods Differential centrifugation Filtration ExoQuick Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%
13 Experiment ID 821 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - exosomes incubated at 25°C Isolation/purification methods Filtration Ultracentrifugation Sucrose and deuterium oxide density cushion Flotation density 1.21 g/mL Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%
14 Experiment ID 822 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - exosomes incubated at 37°C Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.21 g/mL Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%
15 Experiment ID 823 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - exosomes incubated at 42°C Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.21 g/mL Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%
16 Experiment ID 824 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - exosomes at pH 7.1 - Rep 1 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.21 g/mL Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%
17 Experiment ID 825 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - exosomes at pH 7.1 - Rep 2 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.21 g/mL Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%
18 Experiment ID 826 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - exosomes at pH 7.4 - Rep 1 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.21 g/mL Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%
19 Experiment ID 827 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - exosomes at pH 7.4 - Rep 2 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.21 g/mL Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%
20 Experiment ID 828 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 23376832    Organism Rattus norvegicus Experiment description Cardiac myocyte exosomes: stability, HSP60, and proteomics Authors Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA. Journal name Am J Physiol Heart Circ Physiol Publication year 2013 Sample Cardiomyocytes Sample name Cardiomyocyte cells - control Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.21 g/mL Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK EV130100: EV-METRIC:38%, 13%