Gene description for CD81
Gene name	CD81 molecule
Gene symbol	CD81
Other names/aliases	CVID6 S5.7 TAPA1 TSPAN28
Species	Homo sapiens
Experiment description of studies that identified CD81 in Monocytic leukemia cells
1 Experiment ID 1670 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Flow cytometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Monocytic leukemia cells Sample name THP-1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry EV-TRACK -
2 Experiment ID 2502 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Flow cytometry PubMed ID 34322205    Organism Homo sapiens Experiment description Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin Authors Veerman RE, Teeuwen L, Czarnewski P, Güclüler Akpinar G, Sandberg A, Cao X, Pernemalm M, Orre LM, Gabrielsson S, Eldh M. Journal name J Extracell Vesicles Publication year 2021 Sample Monocytic leukemia cells Sample name MM6 - ExoQuick Ultra Isolation/purification methods ExoQuick Kit Flotation density - Molecules identified in the study Protein Methods used in the study Flow cytometry Mass spectometry EV-TRACK -
3 Experiment ID 2503 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Flow cytometry PubMed ID 34322205    Organism Homo sapiens Experiment description Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin Authors Veerman RE, Teeuwen L, Czarnewski P, Güclüler Akpinar G, Sandberg A, Cao X, Pernemalm M, Orre LM, Gabrielsson S, Eldh M. Journal name J Extracell Vesicles Publication year 2021 Sample Monocytic leukemia cells Sample name MM6 - exoEasy Isolation/purification methods exoEasy Maxi Kit Flotation density - Molecules identified in the study Protein Methods used in the study Flow cytometry Mass spectometry EV-TRACK -
4 Experiment ID 2504 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Flow cytometry PubMed ID 34322205    Organism Homo sapiens Experiment description Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin Authors Veerman RE, Teeuwen L, Czarnewski P, Güclüler Akpinar G, Sandberg A, Cao X, Pernemalm M, Orre LM, Gabrielsson S, Eldh M. Journal name J Extracell Vesicles Publication year 2021 Sample Monocytic leukemia cells Sample name MM6 - iZON 35 nm column Isolation/purification methods Size exclusion chromatography Centrifugal ultrafiltration Flotation density - Molecules identified in the study Protein Methods used in the study Flow cytometry Mass spectometry EV-TRACK -
5 Experiment ID 2505 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Large EVs Identification method Flow cytometry PubMed ID 34322205    Organism Homo sapiens Experiment description Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin Authors Veerman RE, Teeuwen L, Czarnewski P, Güclüler Akpinar G, Sandberg A, Cao X, Pernemalm M, Orre LM, Gabrielsson S, Eldh M. Journal name J Extracell Vesicles Publication year 2021 Sample Monocytic leukemia cells Sample name MM6 - iZON 70 nm column Isolation/purification methods Size exclusion chromatography Centrifugal ultrafiltration Flotation density - Molecules identified in the study Protein Methods used in the study Flow cytometry Mass spectometry EV-TRACK -
6 Experiment ID 2506 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Flow cytometry PubMed ID 34322205    Organism Homo sapiens Experiment description Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin Authors Veerman RE, Teeuwen L, Czarnewski P, Güclüler Akpinar G, Sandberg A, Cao X, Pernemalm M, Orre LM, Gabrielsson S, Eldh M. Journal name J Extracell Vesicles Publication year 2021 Sample Monocytic leukemia cells Sample name MM6 - OptiPrep Isolation/purification methods OptiPrep density gradient centrifugation Ultracentrifugation Flotation density 1.12–1.19 g/mL Molecules identified in the study Protein Methods used in the study Flow cytometry Mass spectometry EV-TRACK -
7 Experiment ID 2507 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Flow cytometry PubMed ID 34322206    Organism Homo sapiens Experiment description Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin Authors Veerman RE, Teeuwen L, Czarnewski P, Güclüler Akpinar G, Sandberg A, Cao X, Pernemalm M, Orre LM, Gabrielsson S, Eldh M. Journal name J Extracell Vesicles Publication year 2021 Sample Monocytic leukemia cells Sample name MM6 - MagCapture Isolation/purification methods MagCapture Exosome Isolation Kit PS Flotation density - Molecules identified in the study Protein Methods used in the study Flow cytometry Mass spectometry EV-TRACK -
8 Experiment ID 2508 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Flow cytometry PubMed ID 34322205    Organism Homo sapiens Experiment description Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin Authors Veerman RE, Teeuwen L, Czarnewski P, Güclüler Akpinar G, Sandberg A, Cao X, Pernemalm M, Orre LM, Gabrielsson S, Eldh M. Journal name J Extracell Vesicles Publication year 2021 Sample Monocytic leukemia cells Sample name MM6 - iZON 35 nm column flow-through fraction Isolation/purification methods Size exclusion chromatography Centrifugal ultrafiltration Flotation density - Molecules identified in the study Protein Methods used in the study Flow cytometry Mass spectometry EV-TRACK -
9 Experiment ID 2509 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Flow cytometry PubMed ID 34322205    Organism Homo sapiens Experiment description Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin Authors Veerman RE, Teeuwen L, Czarnewski P, Güclüler Akpinar G, Sandberg A, Cao X, Pernemalm M, Orre LM, Gabrielsson S, Eldh M. Journal name J Extracell Vesicles Publication year 2021 Sample Monocytic leukemia cells Sample name MM6 - iZON 70 nm column flow-through fraction Isolation/purification methods Size exclusion chromatography Centrifugal ultrafiltration Flotation density - Molecules identified in the study Protein Methods used in the study Flow cytometry Mass spectometry EV-TRACK -
10 Experiment ID 2510 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Flow cytometry PubMed ID 34322205    Organism Homo sapiens Experiment description Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin Authors Veerman RE, Teeuwen L, Czarnewski P, Güclüler Akpinar G, Sandberg A, Cao X, Pernemalm M, Orre LM, Gabrielsson S, Eldh M. Journal name J Extracell Vesicles Publication year 2021 Sample Monocytic leukemia cells Sample name MM6 - OptiPrep high density fraction Isolation/purification methods OptiPrep density gradient centrifugation Ultracentrifugation Flotation density Two closest fractions above 1.19 g/mL Molecules identified in the study Protein Methods used in the study Flow cytometry EV-TRACK -
11 Experiment ID 2511 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Flow cytometry PubMed ID 34322205    Organism Homo sapiens Experiment description Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin Authors Veerman RE, Teeuwen L, Czarnewski P, Güclüler Akpinar G, Sandberg A, Cao X, Pernemalm M, Orre LM, Gabrielsson S, Eldh M. Journal name J Extracell Vesicles Publication year 2021 Sample Monocytic leukemia cells Sample name MM6 - OptiPrep low density fraction Isolation/purification methods OptiPrep density gradient centrifugation Ultracentrifugation Flotation density Two closest fractions below 1.12 g/mL Molecules identified in the study Protein Methods used in the study Flow cytometry EV-TRACK -