Gene description for TMEM53
Gene name transmembrane protein 53
Gene symbol TMEM53
Other names/aliases CTDI
NET4
Species Homo sapiens
 Experiment description of studies that identified TMEM53 in Cervical cancer cells

1
Experiment ID1607
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa - CD63 pull-down fraction
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

2
Experiment ID1611
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa - CD9 pull-down fraction
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

3
Experiment ID1613
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

4
Experiment ID1613
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

5
Experiment ID1614
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

6
Experiment ID1614
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

7
Experiment ID1615
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

8
Experiment ID1616
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

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