Gene description for TSG101
Gene name tumor susceptibility 101
Gene symbol TSG101
Other names/aliases TSG10
VPS23
Species Homo sapiens
 Experiment description of studies that identified TSG101 in Urine

1
Experiment ID13
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 15326289   
OrganismHomo sapiens
Experiment descriptionIdentification and proteomic profiling of exosomes in human urine.
AuthorsPisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year2004
SampleUrine
Sample nameUrine - Normal
Isolation/purification methodsUltracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LCQ DECA XP]
Western blotting
EV-TRACK -

2
Experiment ID63
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 19056867   
OrganismHomo sapiens
Experiment descriptionLarge-scale proteomics and phosphoproteomics of urinary exosomes.
AuthorsGonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year2009
SampleUrine
Sample nameUrine - Normal
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
Western blotting
EV-TRACK -

3
Experiment ID382
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 18373357   
OrganismHomo sapiens
Experiment descriptionIsolation and identification of potential urinary microparticle biomarkers of bladder cancer.
AuthorsSmalley DM, Sheman NE, Nelson K, Theodorescu D.
Journal name J Proteome Res
Publication year2008
SampleUrine
Sample nameNormal donors-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

4
Experiment ID383
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 18373357   
OrganismHomo sapiens
Experiment descriptionIsolation and identification of potential urinary microparticle biomarkers of bladder cancer.
AuthorsSmalley DM, Sheman NE, Nelson K, Theodorescu D.
Journal name J Proteome Res
Publication year2008
SampleUrine
Sample namePatients of bladder cancer-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

5
Experiment ID390
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane particles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 19158352   
OrganismHomo sapiens
Experiment descriptionCharacterization of PKD protein-positive exosome-like vesicles.
AuthorsHogan MC, Manganelli L, Woollard JR, Masyuk AI, Masyuk TV, Tammachote R, Huang BQ, Leontovich AA, Beito TG, Madden BJ, Charlesworth MC, Torres VE, LaRusso NF, Harris PC, Ward CJ.
Journal name J Am Soc Nephrol
Publication year2009
SampleUrine
Sample namePatients of autosomal recessive polycystic kidney disease-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

6
Experiment ID429
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 21595033   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
AuthorsMoon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year2011
SampleUrine
Sample nameNormal donors-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV110019: EV-METRIC:44%

7
Experiment ID430
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 21595033   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
AuthorsMoon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year2011
SampleUrine
Sample namePatients of basement membrane nephropathy-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV110019: EV-METRIC:44%

8
Experiment ID431
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 21595033   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
AuthorsMoon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year2011
SampleUrine
Sample namePatients of early IgA nephropathy-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV110019: EV-METRIC:44%

9
Experiment ID437
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 22106071   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of urine exosomes by multidimensional protein identification technology (MudPIT).
AuthorsWang Z, Hill S, Luther JM, Hachey DL, Schey KL.
Journal name Proteomics
Publication year2012
SampleUrine
Sample nameNormal donors-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120168: EV-METRIC:14%

10
Experiment ID444
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 22418980   
OrganismHomo sapiens
Experiment descriptionA multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
AuthorsRaj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year2012
SampleUrine
Sample nameNormal_high density-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120083: EV-METRIC:22%

11
Experiment ID445
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 22418980   
OrganismHomo sapiens
Experiment descriptionA multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
AuthorsRaj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year2012
SampleUrine
Sample nameNormal_low density-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120083: EV-METRIC:22%

12
Experiment ID478
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23082778   
OrganismHomo sapiens
Experiment descriptionComparative and Targeted Proteomic Analyses of Urinary Microparticles from Bladder Cancer and Hernia Patients.
AuthorsChen CL, Lai YF, Tang P, Chien KY, Yu JS, Tsai CH, Chen HW, Wu CC, Chung T, Hsu CW, Chen CD, Chang YS, Chang PL, Chen YT.
Journal name J Proteome Res
Publication year2012
SampleUrine
Sample namePatients of bladder cancer-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120030: EV-METRIC:33%

13
Experiment ID479
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23082778   
OrganismHomo sapiens
Experiment descriptionComparative and Targeted Proteomic Analyses of Urinary Microparticles from Bladder Cancer and Hernia Patients.
AuthorsChen CL, Lai YF, Tang P, Chien KY, Yu JS, Tsai CH, Chen HW, Wu CC, Chung T, Hsu CW, Chen CD, Chang YS, Chang PL, Chen YT.
Journal name J Proteome Res
Publication year2012
SampleUrine
Sample namePatients of hernia-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120030: EV-METRIC:33%

14
Experiment ID498
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23376485   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of podocyte exosome-enriched fraction from normal human urine.
AuthorsPrunotto M, Farina A, Lane L, Pernin A, Schifferli J, Hochstrasser DF, Lescuyer P, Moll S.
Journal name J Proteomics
Publication year2013
SampleUrine
Sample nameNormal-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130051: EV-METRIC:38%

15
Experiment ID507
Identified moleculeprotein
Extracellular vesicle typeExosomes/Nanovesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23511837   
OrganismHomo sapiens
Experiment descriptionDifferential protein profiling of renal cell carcinoma urinary exosomes.
AuthorsRaimondo F, Morosi L, Corbetta S, Chinello C, Brambilla P, Della Mina P, Villa A, Albo G, Battaglia C, Bosari S, Magni F, Pitto M.
Journal name Mol Biosyst
Publication year2013
SampleUrine
Sample nameNormal-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130016: EV-METRIC:44%

16
Experiment ID508
Identified moleculeprotein
Extracellular vesicle typeExosomes/Nanovesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23511837   
OrganismHomo sapiens
Experiment descriptionDifferential protein profiling of renal cell carcinoma urinary exosomes.
AuthorsRaimondo F, Morosi L, Corbetta S, Chinello C, Brambilla P, Della Mina P, Villa A, Albo G, Battaglia C, Bosari S, Magni F, Pitto M.
Journal name Mol Biosyst
Publication year2013
SampleUrine
Sample nameRenal cell carcinoma patient-Urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130016: EV-METRIC:44%

17
Experiment ID512
Identified moleculeprotein
Extracellular vesicle typeExosomes/Microvesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23533145   
OrganismHomo sapiens
Experiment descriptionIn-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
AuthorsPrincipe S, Jones EE, Kim Y, Sinha A, Nyalwidhe JO, Brooks J, Semmes OJ, Troyer DA, Lance RS, Kislinger T, Drake RR.
Journal name Proteomics
Publication year2013
SampleUrine
Sample nameLow-grade prostate cancer patient-Expressed prostatic secretions in urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130224: EV-METRIC:14%

18
Experiment ID513
Identified moleculeprotein
Extracellular vesicle typeExosomes/Microvesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23533145   
OrganismHomo sapiens
Experiment descriptionIn-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
AuthorsPrincipe S, Jones EE, Kim Y, Sinha A, Nyalwidhe JO, Brooks J, Semmes OJ, Troyer DA, Lance RS, Kislinger T, Drake RR.
Journal name Proteomics
Publication year2013
SampleUrine
Sample nameNormal-Expressed prostatic secretions in urine
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130224: EV-METRIC:14%

19
Experiment ID1187
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method ELISA
PubMed ID 28326167   
OrganismHomo sapiens
Experiment descriptionResidual urinary extracellular vesicles in ultracentrifugation supernatants after hydrostatic filtration dialysis enrichment.
AuthorsMusante L, Tataruch-Weinert D, Kerjaschki D, Henry M, Meleady P, Holthofer H.
Journal name J Extracell Vesicles.
Publication year2016
SampleUrine
Sample namePelelt, HFDa P200
Isolation/purification methodsHydrostatic filtration dialysis
Differential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

20
Experiment ID1188
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method ELISA
PubMed ID 28326167   
OrganismHomo sapiens
Experiment descriptionResidual urinary extracellular vesicles in ultracentrifugation supernatants after hydrostatic filtration dialysis enrichment.
AuthorsMusante L, Tataruch-Weinert D, Kerjaschki D, Henry M, Meleady P, Holthofer H.
Journal name J Extracell Vesicles.
Publication year2016
SampleUrine
Sample namePelelt, HFDa SN200
Isolation/purification methodsHydrostatic filtration dialysis
Differential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

21
Experiment ID1186
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method ELISA
Western blotting
PubMed ID 28326167   
OrganismHomo sapiens
Experiment descriptionResidual urinary extracellular vesicles in ultracentrifugation supernatants after hydrostatic filtration dialysis enrichment.
AuthorsMusante L, Tataruch-Weinert D, Kerjaschki D, Henry M, Meleady P, Holthofer H.
Journal name J Extracell Vesicles.
Publication year2016
SampleUrine
Sample namePelelt, HFDa P40
Isolation/purification methodsHydrostatic filtration dialysis
Differential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

22
Experiment ID725
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 25471207   
OrganismHomo sapiens
Experiment descriptionIntraluminal proteome and peptidome of human urinary extracellular vesicles.
AuthorsLiu X, Chinello C, Musante L, Cazzaniga M, Tataruch D, Calzaferri G, James Smith A, De Sio G, Magni F, Zou H, Holthofer H.
Journal name Proteomics Clin Appl.
Publication year2015
SampleUrine
Sample nameNormal crude urine- Trypsinized
Isolation/purification methodsDifferential centrifugation
Hydrostatic filtration dialysis
Ultrafiltration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV140189: EV-METRIC:25%

23
Experiment ID751
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 29988836   
OrganismHomo sapiens
Experiment descriptionUltrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine
AuthorsOeyen E, Van Mol K, Baggerman G, Willems H, Boonen K, Rolfo C, Pauwels P, Jacobs A, Schildermans K, Cho WC, Mertens I.
Journal name J Extracell Vesicles
Publication year2018
SampleUrine
Sample nameUrine - Fraction 8 and 9 pooled
Isolation/purification methodsFiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170025: EV-METRIC:62%

24
Experiment ID1161
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 22641613   
OrganismHomo sapiens
Experiment descriptionApplication of systems biology principles to protein biomarker discovery: urinary exosomal proteome in renal transplantation.
AuthorsPisitkun T, Gandolfo MT, Das S, Knepper MA, Bagnasco SM.
Journal name Proteomics Clin Appl
Publication year2012
SampleUrine
Sample nameRenal allograft recipient with cell-mediated rejection
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120152: EV-METRIC:14%

25
Experiment ID724
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 25471207   
OrganismHomo sapiens
Experiment descriptionIntraluminal proteome and peptidome of human urinary extracellular vesicles.
AuthorsLiu X, Chinello C, Musante L, Cazzaniga M, Tataruch D, Calzaferri G, James Smith A, De Sio G, Magni F, Zou H, Holthofer H.
Journal name Proteomics Clin Appl.
Publication year2015
SampleUrine
Sample nameNormal crude urine
Isolation/purification methodsDifferential centrifugation
Hydrostatic filtration dialysis
Ultrafiltration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV140189: EV-METRIC:25%

26
Experiment ID958
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 26196085   
OrganismHomo sapiens
Experiment descriptionIdentification of prostate cancer biomarkers in urinary exosomes.
Authors"Overbye A, Skotland T, Koehler CJ, Thiede B, Seierstad T, Berge V, Sandvig K, Llorente A.
Journal name Oncotarget
Publication year2015
SampleUrine
Sample nameUrine - prostate cancer control
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV150029: EV-METRIC:33%

27
Experiment ID1027
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 24211404   
OrganismHomo sapiens
Experiment descriptionDiabetic nephropathy induces changes in the proteome of human urinary exosomes as revealed by label-free comparative analysis.
AuthorsZubiri I, Posada-Ayala M, Sanz-Maroto A, Calvo E, Martin-Lorenzo M, Gonzalez-Calero L, de la Cuesta F, Lopez JA, Fernandez-Fernandez B, Ortiz A, Vivanco F, Alvarez-Llamas G.
Journal name J Proteomics.
Publication year2014
SampleUrine
Sample nameUrine - Diabetic nephropathy (DN) patients
Isolation/purification methodsUltracentrifugation
ExoQuick
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV140245: EV-METRIC:22%, 25%, 22%

28
Experiment ID1189
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 28326167   
OrganismHomo sapiens
Experiment descriptionResidual urinary extracellular vesicles in ultracentrifugation supernatants after hydrostatic filtration dialysis enrichment.
AuthorsMusante L, Tataruch-Weinert D, Kerjaschki D, Henry M, Meleady P, Holthofer H.
Journal name J Extracell Vesicles.
Publication year2016
SampleUrine
Sample namePelelt, HFDa
Isolation/purification methodsHydrostatic filtration dialysis
Differential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

29
Experiment ID1272
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 24058411   
OrganismHomo sapiens
Experiment descriptionOsteoprotegerin in exosome-like vesicles from human cultured tubular cells and urine.
AuthorsBenito-Martin A, Ucero AC, Zubiri I, Posada-Ayala M, Fernandez-Fernandez B, Cannata-Ortiz P, Sanchez-Nino MD, Ruiz-Ortega M, Egido J, Alvarez-Llamas G, Ortiz A.
Journal name PLoS One.
Publication year2013
SampleUrine
Sample nameHealthy volunteers
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV130007: EV-METRIC:44%, 44%

30
Experiment ID1273
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 24058411   
OrganismHomo sapiens
Experiment descriptionOsteoprotegerin in exosome-like vesicles from human cultured tubular cells and urine.
AuthorsBenito-Martin A, Ucero AC, Zubiri I, Posada-Ayala M, Fernandez-Fernandez B, Cannata-Ortiz P, Sanchez-Nino MD, Ruiz-Ortega M, Egido J, Alvarez-Llamas G, Ortiz A.
Journal name PLoS One.
Publication year2013
SampleUrine
Sample namechronic kidney disease patients
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV130007: EV-METRIC:44%, 44%

31
Experiment ID2754
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample name10 -kPa
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

32
Experiment ID2755
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample name20 -kPa
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

33
Experiment ID2756
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample name30 -kPa
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

34
Experiment ID2757
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample name40 -kPa
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

35
Experiment ID2758
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample name50 -kPa
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

36
Experiment ID2759
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameUrine
Isolation/purification methodsTangential flow filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

37
Experiment ID2760
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample name10 sec conversion time
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

38
Experiment ID2761
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample name20 sec conversion time
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

39
Experiment ID2762
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample name30 sec conversion time
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

40
Experiment ID2763
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample name40 sec conversion time
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

41
Experiment ID2764
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample name50 sec conversion time
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

42
Experiment ID2765
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameUrine
Isolation/purification methodsTangential flow filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

43
Experiment ID2766
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameUrine
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

44
Experiment ID2767
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameUrine
Isolation/purification methodsFiltration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

45
Experiment ID2768
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameUrine
Isolation/purification methodsFiltration
Polyethylene glycol-based precipitation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

46
Experiment ID2769
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameUrine
Isolation/purification methodsFiltration
Membrane affinity purification
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

47
Experiment ID2770
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameUrine
Isolation/purification methodsFiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

48
Experiment ID2771
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameUrine
Isolation/purification methodsFiltration
Phosphatidylserine affinity purification
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

49
Experiment ID2775
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameFresh
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

50
Experiment ID2776
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameStored at 4°C for 2 days
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

51
Experiment ID2777
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33432243   
OrganismHomo sapiens
Experiment descriptionExosome detection via the ultrafast-isolation system: EXODUS
AuthorsChen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F.
Journal name Nat Methods
Publication year2021
SampleUrine
Sample nameStored at -80°C
Isolation/purification methodsFiltration
Exosome detection via ultrafast-isolation system (EXODUS)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

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