Gene description for FLOT2
Gene name	flotillin 2
Gene symbol	FLOT2
Other names/aliases	ECS-1 ECS1 ESA ESA1 M17S1
Species	Homo sapiens
Experiment description of studies that identified FLOT2 in Breast cancer cells
1 Experiment ID 210 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Western blotting PubMed ID 21368175    Organism Homo sapiens Experiment description Cancer cell-derived microvesicles induce transformation by transferring tissue transglutaminase and fibronectin to recipient cells Authors Antonyak MA, Li B, Boroughs LK, Johnson JL, Druso JE, Bryant KL, Holowka DA, Cerione RA Journal name Proc Natl Acad Sci U S A Publication year 2011 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Immunofluorescent microscopy Scanning electron microscopy Mass spectrometry [4000 Q Trap] EV-TRACK -
2 Experiment ID 575 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Breast cancer cells Sample name BT549 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
3 Experiment ID 579 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Breast cancer cells Sample name MDA-MB-468 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
4 Experiment ID 1957 Identified molecule protein Extracellular vesicle type Microvesicles MISEV Large EVs Identification method Western blotting PubMed ID 36475545    Organism Homo sapiens Experiment description Weakly migratory metastatic breast cancer cells activate fibroblasts via microvesicle-Tg2 to facilitate dissemination and metastasis Authors Schwager SC, Young KM, Hapach LA, Carlson CM, Mosier JA, McArdle TJ, Wang W, Schunk C, Jayathilake AL, Bates ME, Bordeleau F, Antonyak MA, Cerione RA, Reinhart-King CA. Journal name Elife Publication year 2022 Sample Breast cancer cells Sample name MDA-MB-231 - weakly migratory (MDA- ) Isolation/purification methods Centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry EV-TRACK -
5 Experiment ID 1958 Identified molecule protein Extracellular vesicle type Microvesicles MISEV Large EVs Identification method Western blotting PubMed ID 36475545    Organism Homo sapiens Experiment description Weakly migratory metastatic breast cancer cells activate fibroblasts via microvesicle-Tg2 to facilitate dissemination and metastasis Authors Schwager SC, Young KM, Hapach LA, Carlson CM, Mosier JA, McArdle TJ, Wang W, Schunk C, Jayathilake AL, Bates ME, Bordeleau F, Antonyak MA, Cerione RA, Reinhart-King CA. Journal name Elife Publication year 2022 Sample Breast cancer cells Sample name MDA-MB-231 - highly migratory (MDA+) Isolation/purification methods Centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry EV-TRACK -
6 Experiment ID 1959 Identified molecule protein Extracellular vesicle type Microvesicles MISEV Large EVs Identification method Western blotting PubMed ID 36475545    Organism Homo sapiens Experiment description Weakly migratory metastatic breast cancer cells activate fibroblasts via microvesicle-Tg2 to facilitate dissemination and metastasis Authors Schwager SC, Young KM, Hapach LA, Carlson CM, Mosier JA, McArdle TJ, Wang W, Schunk C, Jayathilake AL, Bates ME, Bordeleau F, Antonyak MA, Cerione RA, Reinhart-King CA. Journal name Elife Publication year 2022 Sample Breast cancer cells Sample name MDA-MB-231 - weakly migratory (MDA- ) Isolation/purification methods Centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry EV-TRACK -
7 Experiment ID 1960 Identified molecule protein Extracellular vesicle type Microvesicles MISEV Large EVs Identification method Western blotting PubMed ID 36475545    Organism Homo sapiens Experiment description Weakly migratory metastatic breast cancer cells activate fibroblasts via microvesicle-Tg2 to facilitate dissemination and metastasis Authors Schwager SC, Young KM, Hapach LA, Carlson CM, Mosier JA, McArdle TJ, Wang W, Schunk C, Jayathilake AL, Bates ME, Bordeleau F, Antonyak MA, Cerione RA, Reinhart-King CA. Journal name Elife Publication year 2022 Sample Breast cancer cells Sample name MDA-MB-231 - weakly migratory (MDA- ) Isolation/purification methods Centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry EV-TRACK -
8 Experiment ID 1664 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MCF7 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry EV-TRACK -
9 Experiment ID 1666 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry EV-TRACK -
10 Experiment ID 1678 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
11 Experiment ID 1678 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
12 Experiment ID 1679 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -