Gene description for ERBB2
Gene name	erb-b2 receptor tyrosine kinase 2
Gene symbol	ERBB2
Other names/aliases	CD340 HER-2 HER-2/neu HER2 MLN 19 MLN-19 NEU NGL TKR1 VSCN2 c-ERB-2 c-ERB2 p185(erbB2)
Species	Homo sapiens
Experiment description of studies that identified ERBB2 in Breast cancer cells
1 Experiment ID 19 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting FACS PubMed ID 16302729    Organism Homo sapiens Experiment description Purification, characterization and biological significance of tumor-derived exosomes. Authors Koga K, Matsumoto K, Akiyoshi T, Kubo M, Yamanaka N, Tasaki A, Nakashima H, Nakamura M, Kuroki S, Tanaka M, Katano M Journal name ACR Publication year 2005 Sample Breast cancer cells Sample name BT-474 MDA-MB-231 Isolation/purification methods Differential centrifugation Sucrose density gradient Filtration Immunobeads(HER2) Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting FACS EV-TRACK -
2 Experiment ID 216 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Imuunoelectron microscopy FACS Western blotting Dot blot PubMed ID 21293856    Organism Homo sapiens Experiment description Tumour exosomes inhibit binding of tumour-reactive antibodies to tumour cells and reduce ADCC Authors Battke C, Ruiss R, Welsch U, Wimberger P, Lang S, Jochum S, Zeidler R Journal name Cancer Immunol Immunother Publication year 2011 Sample Breast cancer cells Sample name BT474 Isolation/purification methods Differential centrifugation Filtration Ultrafiltration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.17 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Immunoelectron microscopy FACS Dot blot EV-TRACK EV110080: EV-METRIC:14%, 14%, 38%
3 Experiment ID 579 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Breast cancer cells Sample name MDA-MB-468 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
4 Experiment ID 1029 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 26378940    Organism Homo sapiens Experiment description Redefining the Breast Cancer Exosome Proteome by Tandem Mass Tag Quantitative Proteomics and Multivariate Cluster Analysis. Authors Clark DJ, Fondrie WE, Liao Z, Hanson PI, Fulton A, Mao L, Yang AJ. Journal name Anal Chem. Publication year 2015 Sample Breast cancer cells Sample name SKBR3B - 100 K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV150004: EV-METRIC:67%, 38%
5 Experiment ID 3514 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Thermophoretic aptasensor (TAS) PubMed ID 30948809    Organism Homo sapiens Experiment description Low-cost thermophoretic profiling of extracellular-vesicle surface proteins for the early detection and classification of cancers Authors Liu C, Zhao J, Tian F, Cai L, Zhang W, Feng Q, Chang J, Wan F, Yang Y, Dai B, Cong Y, Ding B, Sun J, Tan W. Journal name Nat Biomed Eng Publication year 2019 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Thermophoretic aptasensor (TAS) EV-TRACK -