Gene description for PDIA3
Gene name protein disulfide isomerase family A member 3
Gene symbol PDIA3
Other names/aliases grp-58
Species Sus scrofa
 Experiment description of studies that identified PDIA3 in Polarised retinal pigmented epithelial cells

1
Experiment ID916
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28687758   
OrganismSus scrofa
Experiment descriptionDirectional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers
AuthorsKlingeborn M, Dismuke WM, Skiba NP, Kelly U, Stamer WD, Bowes Rickman C.
Journal name Sci Rep
Publication year2017
SamplePolarised retinal pigmented epithelial cells
Sample namePolarised RPE cells - apical - Rep 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.07-1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170046: EV-METRIC:88%, 88%

2
Experiment ID917
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28687758   
OrganismSus scrofa
Experiment descriptionDirectional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers
AuthorsKlingeborn M, Dismuke WM, Skiba NP, Kelly U, Stamer WD, Bowes Rickman C.
Journal name Sci Rep
Publication year2017
SamplePolarised retinal pigmented epithelial cells
Sample namePolarised RPE cells - apical - Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.07-1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170046: EV-METRIC:88%, 88%

3
Experiment ID919
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28687758   
OrganismSus scrofa
Experiment descriptionDirectional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers
AuthorsKlingeborn M, Dismuke WM, Skiba NP, Kelly U, Stamer WD, Bowes Rickman C.
Journal name Sci Rep
Publication year2017
SamplePolarised retinal pigmented epithelial cells
Sample namePolarised RPE cells - basolateral - Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.07-1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170046: EV-METRIC:88%, 88%

4
Experiment ID920
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28687758   
OrganismSus scrofa
Experiment descriptionDirectional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers
AuthorsKlingeborn M, Dismuke WM, Skiba NP, Kelly U, Stamer WD, Bowes Rickman C.
Journal name Sci Rep
Publication year2017
SamplePolarised retinal pigmented epithelial cells
Sample namePolarised RPE cells - apical - Fraction 9
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170046: EV-METRIC:88%, 88%

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