Gene description for GNAS
Gene name GNAS complex locus
Gene symbol GNAS
Other names/aliases NESP55
PORGSA1
SCG6
Species Sus scrofa
 Experiment description of studies that identified GNAS in Polarised retinal pigmented epithelial cells

1
Experiment ID916
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28687758   
OrganismSus scrofa
Experiment descriptionDirectional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers
AuthorsKlingeborn M, Dismuke WM, Skiba NP, Kelly U, Stamer WD, Bowes Rickman C.
Journal name Sci Rep
Publication year2017
SamplePolarised retinal pigmented epithelial cells
Sample namePolarised RPE cells - apical - Rep 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.07-1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170046: EV-METRIC:88%, 88%

2
Experiment ID917
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28687758   
OrganismSus scrofa
Experiment descriptionDirectional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers
AuthorsKlingeborn M, Dismuke WM, Skiba NP, Kelly U, Stamer WD, Bowes Rickman C.
Journal name Sci Rep
Publication year2017
SamplePolarised retinal pigmented epithelial cells
Sample namePolarised RPE cells - apical - Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.07-1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170046: EV-METRIC:88%, 88%

3
Experiment ID919
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28687758   
OrganismSus scrofa
Experiment descriptionDirectional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers
AuthorsKlingeborn M, Dismuke WM, Skiba NP, Kelly U, Stamer WD, Bowes Rickman C.
Journal name Sci Rep
Publication year2017
SamplePolarised retinal pigmented epithelial cells
Sample namePolarised RPE cells - basolateral - Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.07-1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170046: EV-METRIC:88%, 88%

4
Experiment ID920
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28687758   
OrganismSus scrofa
Experiment descriptionDirectional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers
AuthorsKlingeborn M, Dismuke WM, Skiba NP, Kelly U, Stamer WD, Bowes Rickman C.
Journal name Sci Rep
Publication year2017
SamplePolarised retinal pigmented epithelial cells
Sample namePolarised RPE cells - apical - Fraction 9
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170046: EV-METRIC:88%, 88%

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