Results shown for selected experiment:


Experiment ID38
PubMed ID 33991177   
Identification method Protein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
SpeciesHomo sapiens
Experiment descriptionCancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
SampleGastric cancer cells
Sample nameMKN1 - 100K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Methods used in the studyWestern blotting
Mass spectrometry
Flotation density-
AuthorsCarli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year2021
EV-TRACK -



   Contents identified in selected experiment:





   Top 200 abundant gene(s) for selected experiment:




   Other experiments from same study (PubMed ID):



 PubMed IDSpeciesExperiment descriptionSampleSample nameIdentificationsIsolation methodsVesicle typeMISEVQuantification
1 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation
Small extracellular vesicles Small EVs EVQuant
2 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation
Small extracellular vesicles Small EVs EVQuant
3 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation
Small extracellular vesicles Small EVs EVQuant



   Experiments in which FEN1 is identified:



 PubMed IDSpeciesExperiment descriptionSampleSample nameIdentificationsIsolation methodsVesicle typeMISEVQuantification
1 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation
Small extracellular vesicles Small EVs EVQuant
2 33661579 Homo sapiens Pannexin-1 channel regulates nuclear content packaging into apoptotic bodies and their size T lymphoblast Jurkat T cells Protein Differential centrifugation Apoptotic bodies Large EVs EVQuant
3 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation
Small extracellular vesicles Small EVs EVQuant
4 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Pancreatic cancer cells PANC-1 - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Exosomes Small EVs EVQuant
5 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control129 Protein Centrifugal ultrafiltration
Size exclusion chromatography
Extracellular vesicles Small EVs EVQuant
6 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE128 Protein Centrifugal ultrafiltration
Size exclusion chromatography
Extracellular vesicles Small EVs EVQuant
7 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Breast cancer cells MDA-MB-231 - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Exosomes Small EVs EVQuant
8 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE130 Protein Centrifugal ultrafiltration
Size exclusion chromatography
Extracellular vesicles Small EVs EVQuant
9 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-Cas9-WT Protein Differential centrifugation
Filtration
Ultracentrifugation
Small extracellular vesicles Small EVs EVQuant
10 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Pancreatic cancer cells PANC-1 - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Exosomes Small EVs EVQuant
11 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Embryonic kidney cells HEK293T - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Exosomes Small EVs EVQuant
12 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE131 Protein Centrifugal ultrafiltration
Size exclusion chromatography
Extracellular vesicles Small EVs EVQuant
13 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Breast cancer cells MDA-MB-231 - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Exosomes Small EVs EVQuant
14 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE127 Protein Centrifugal ultrafiltration
Size exclusion chromatography
Extracellular vesicles Small EVs EVQuant
15 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-KO-LAMP2A Protein Differential centrifugation
Filtration
Ultracentrifugation
Small extracellular vesicles Small EVs EVQuant
16 33661579 Homo sapiens Pannexin-1 channel regulates nuclear content packaging into apoptotic bodies and their size T lymphoblast Jurkat T cells Protein Differential centrifugation Apoptotic bodies Large EVs EVQuant
17 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control126 Protein Centrifugal ultrafiltration
Size exclusion chromatography
Extracellular vesicles Small EVs EVQuant
18 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Embryonic kidney cells HEK293T - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Exosomes Small EVs EVQuant
19 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control130 Protein Centrifugal ultrafiltration
Size exclusion chromatography
Extracellular vesicles Small EVs EVQuant
20 33661579 Homo sapiens Pannexin-1 channel regulates nuclear content packaging into apoptotic bodies and their size T lymphoblast Jurkat T cells Protein Differential centrifugation Apoptotic bodies Large EVs EVQuant
21 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE129 Protein Centrifugal ultrafiltration
Size exclusion chromatography
Extracellular vesicles Small EVs EVQuant
22 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation
Small extracellular vesicles Small EVs EVQuant
23 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control127 Protein Centrifugal ultrafiltration
Size exclusion chromatography
Extracellular vesicles Small EVs EVQuant