Results shown for selected experiment:


Experiment ID33
PubMed ID 32938681   
Identification method Protein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
SpeciesHomo sapiens
Experiment descriptionAnnexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis
SampleValvular interstitial cells
Sample nameAortic valve-derived primary valvular interstial cells
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Methods used in the studyMass spectrometry
Western blotting
Flotation density-
AuthorsRogers MA, Buffolo F, Schlotter F, Atkins SK, Lee LH, Halu A, Blaser MC, Tsolaki E, Higashi H, Luther K, Daaboul G, Bouten CVC, Body SC, Singh SA, Bertazzo S, Libby P, Aikawa M, Aikawa E.
Journal name Sci Adv
Publication year2020
EV-TRACK -



   Contents identified in selected experiment:





   Top 200 abundant gene(s) for selected experiment:




   Other experiments from same study (PubMed ID):



 PubMed IDSpeciesExperiment descriptionSampleSample nameIdentificationsIsolation methodsVesicle typeMISEVQuantification
1 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Valvular interstitial cells Aortic valve-derived primary valvular interstial cells Protein Differential centrifugation
Ultracentrifugation
Extracellular vesicles EVs EVQuant
2 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Smooth muscle cells Carotid artery-derived primary smooth muscle cells Protein Differential centrifugation
Ultracentrifugation
Extracellular vesicles EVs EVQuant
3 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Smooth muscle cells Carotid artery-derived primary smooth muscle cells Protein Differential centrifugation
Ultracentrifugation
Extracellular vesicles EVs EVQuant
4 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Valvular interstitial cells Aortic valve-derived primary valvular interstial cells Protein Differential centrifugation
Ultracentrifugation
Extracellular vesicles EVs EVQuant



   Experiments in which SPTBN1 is identified:



 PubMed IDSpeciesExperiment descriptionSampleSample nameIdentificationsIsolation methodsVesicle typeMISEVQuantification
1 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation
Small extracellular vesicles Small EVs EVQuant
2 33661579 Homo sapiens Pannexin-1 channel regulates nuclear content packaging into apoptotic bodies and their size T lymphoblast Jurkat T cells Protein Differential centrifugation Apoptotic bodies Large EVs EVQuant
3 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation
Small extracellular vesicles Small EVs EVQuant
4 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Pancreatic cancer cells PANC-1 - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Exosomes Small EVs EVQuant
5 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control129 Protein Centrifugal ultrafiltration
Size exclusion chromatography
Extracellular vesicles Small EVs EVQuant
6 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE128 Protein Centrifugal ultrafiltration
Size exclusion chromatography
Extracellular vesicles Small EVs EVQuant
7 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Valvular interstitial cells Aortic valve-derived primary valvular interstial cells Protein Differential centrifugation
Ultracentrifugation
Extracellular vesicles EVs EVQuant
8 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Breast cancer cells MDA-MB-231 - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Exosomes Small EVs EVQuant
9 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE130 Protein Centrifugal ultrafiltration
Size exclusion chromatography
Extracellular vesicles Small EVs EVQuant
10 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Valvular interstitial cells Aortic valve-derived primary valvular interstial cells Protein Differential centrifugation
Ultracentrifugation
Extracellular vesicles EVs EVQuant
11 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-Cas9-WT Protein Differential centrifugation
Filtration
Ultracentrifugation
Small extracellular vesicles Small EVs EVQuant
12 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Pancreatic cancer cells PANC-1 - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Exosomes Small EVs EVQuant
13 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Embryonic kidney cells HEK293T - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Exosomes Small EVs EVQuant
14 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE131 Protein Centrifugal ultrafiltration
Size exclusion chromatography
Extracellular vesicles Small EVs EVQuant
15 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Smooth muscle cells Carotid artery-derived primary smooth muscle cells Protein Differential centrifugation
Ultracentrifugation
Extracellular vesicles EVs EVQuant
16 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Breast cancer cells MDA-MB-231 - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Exosomes Small EVs EVQuant
17 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE127 Protein Centrifugal ultrafiltration
Size exclusion chromatography
Extracellular vesicles Small EVs EVQuant
18 35333565 Homo sapiens LAMP2A regulates the loading of proteins into exosomes Retinal pigment epithelial cells ARPE-19-KO-LAMP2A Protein Differential centrifugation
Filtration
Ultracentrifugation
Small extracellular vesicles Small EVs EVQuant
19 33661579 Homo sapiens Pannexin-1 channel regulates nuclear content packaging into apoptotic bodies and their size T lymphoblast Jurkat T cells Protein Differential centrifugation Apoptotic bodies Large EVs EVQuant
20 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control126 Protein Centrifugal ultrafiltration
Size exclusion chromatography
Extracellular vesicles Small EVs EVQuant
21 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Embryonic kidney cells HEK293T - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Exosomes Small EVs EVQuant
22 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control130 Protein Centrifugal ultrafiltration
Size exclusion chromatography
Extracellular vesicles Small EVs EVQuant
23 32938681 Homo sapiens Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis Smooth muscle cells Carotid artery-derived primary smooth muscle cells Protein Differential centrifugation
Ultracentrifugation
Extracellular vesicles EVs EVQuant
24 33661579 Homo sapiens Pannexin-1 channel regulates nuclear content packaging into apoptotic bodies and their size T lymphoblast Jurkat T cells Protein Differential centrifugation Apoptotic bodies Large EVs EVQuant
25 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells CSE129 Protein Centrifugal ultrafiltration
Size exclusion chromatography
Extracellular vesicles Small EVs EVQuant
26 33991177 Homo sapiens Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Gastric cancer cells MKN1 - 100K pellet Protein Differential centrifugation
Ultracentrifugation
Small extracellular vesicles Small EVs EVQuant
27 30863515 Homo sapiens Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles Lung epithelial cells Control127 Protein Centrifugal ultrafiltration
Size exclusion chromatography
Extracellular vesicles Small EVs EVQuant